Protocol 2

NoteQuick summary:

More in-depth and feature-focused protocol. This protocol typically takes between 20-25 minutes.

• Aim 1: Determine higher resolution features of the cell’s receptive field by using smaller flashes.
• Aim 2: Determine whether the cell displays directionally selective responses to bar sweeps in 16 directions.

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• Overview:

• Longer, high resolution probing of the cell’s receptive field.

• Custom script design.

• Opens a pop-up at the beginning that asks the user to fill in the peak_frame from P1 and the side of the arena used for P1.

• This peak_frame value is then used to generate a protocol with the stimuli centred on the centre of the flash that elicited the greatest response in P1.

• These stimuli are presented within a 30 x 30 pixel area. The rest of the arena is set to the background intensity value.

• Only presents either dark or light stimuli. However, at the end of the protocol the peak_frame value that generates the protocol at the same location with the inverse contrast is returned so that the protocol can be run twice, once for each contrast if desired.

• Location: P2 is made de novo every time the function generate_protocol2() is run. A new folder of the format ‘yyyy_MM_dd_HH_mm’ is made within the the folder C:\matlabroot\G4_Protocols\nested_RF_protocol2 that contains both the patterns, functions and experiment file currentExp.mat and the data that was recorded in the Log file.

Stimulus parameters:

Protocol design:

Flash presentation:

Figure 1 - Protocol 2 stimuli: Single dark 4 x 4 pixel flash (left) and 4 pixel wide bar (right) as presented in P2 on the G4 LED arena. These stimuli are presented within a 30 x 30 pixel area centred on the peak frame from P1.