From October 2025, automatic logging to a google sheet was implemented. This google sheet contains the full experiment log from this date onwards.
tutl RNAi experiments
The original experiments with the nested_RF_protocol.
Fly strains
| w;pJFRC28-10XUAS-IVS-GFP-p10 (su(Hw)attP5);GR_42F06 |
y,sc,v,sev; P{TRiP HMJ21587}(attP40);+ |
T4/T5 |
tutl RNAi, cytoplasmic GFP |
PC = Preferred Contrast determined from Protocol 1 (P1). Either ON (light flash) or OFF (dark flash).
Experiment log
| 2024_12_12 |
12_12 |
RNAi_ttl_2025/control/ON/2024_12_12_12_12 |
control |
ON |
| 2024_12_12 |
13_40 |
RNAi_ttl_2025/control/OFF/2024_12_12_13_40 |
control |
OFF |
| 2024_12_18 |
15_07 |
RNAi_ttl_2025/control/ON/2024_12_18_15_07 |
control |
ON |
| 2024_12_18 |
15_49 |
RNAi_ttl_2025/control/OFF/2024_12_18_15_49 |
control |
OFF |
| 2024_12_19 |
15_53 |
RNAi_ttl_2025/control/OFF/2024_12_19_15_53 |
control |
OFF |
| 2024_12_20 |
09_57 |
RNAi_ttl_2025/control/OFF/2024_12_20_09_57 |
control |
OFF |
| 2024_12_20 |
10_45 |
RNAi_ttl_2025/control/OFF/2024_12_20_10_45 |
control |
OFF |
| 2024_12_20 |
17_00 |
RNAi_ttl_2025/control/OFF/2024_12_20_17_00 |
control |
OFF |
| 2024_12_23 |
11_31 |
RNAi_ttl_2025/ttl/OFF/2024_12_23_11_31 |
ttl |
OFF |
| 2024_12_23 |
12_28 |
RNAi_ttl_2025/ttl/OFF/2024_12_23_12_28 |
ttl |
OFF |
| 2024_12_24 |
13_21 |
RNAi_ttl_2025/ttl/OFF/2024_12_24_13_21 |
ttl |
OFF |
| 2024_12_24 |
16_50 |
RNAi_ttl_2025/ttl/OFF/2024_12_24_16_50 |
ttl |
OFF |
| 2025_01_02 |
14_05 |
RNAi_ttl_2025/control/OFF/2025_01_02_14_05 |
control |
OFF |
| 2025_01_03 |
13_12 |
RNAi_ttl_2025/ttl/ON/2025_01_03_13_12 |
ttl |
ON |
| 2025_01_03 |
14_24 |
RNAi_ttl_2025/ttl/OFF/2025_01_03_14_24 |
ttl |
OFF |
| 2025_01_10 |
12_24 |
RNAi_ttl_2025/ttl/OFF/2025_01_10_12_24 |
ttl |
OFF |
| 2025_01_13 |
15_40 |
RNAi_ttl_2025/ttl/OFF/2025_01_13_15_40 |
ttl |
OFF |
| 2025_01_14 |
15_41 |
RNAi_ttl_2025/control/ON/2025_01_14_15_41 |
control |
ON |
| 2025_01_15 |
12_29 |
RNAi_ttl_2025/control/ON/2025_01_15_12_29 |
control |
ON |
| 2025_01_15 |
17_03 |
RNAi_ttl_2025/control/ON/2025_01_15_17_03 |
control |
ON |
| 2025_01_17 |
12_40 |
RNAi_ttl_2025/ttl/ON/2025_01_17_12_40 |
ttl |
ON |
| 2025_01_17 |
13_31 |
RNAi_ttl_2025/ttl/ON/2025_01_17_13_31 |
ttl |
ON |
| 2025_01_21 |
15_35 |
RNAi_ttl_2025/ttl/ON/2025_01_21_15_35 |
ttl |
ON |
| 2025_01_21 |
16_09 |
RNAi_ttl_2025/ttl/ON/2025_01_21_16_09 |
ttl |
ON |
| 2025_01_22 |
14_34 |
RNAi_ttl_2025/ttl/ON/2025_01_22_14_34 |
ttl |
ON |
| 2025_01_22 |
16_03 |
RNAi_ttl_2025/ttl/ON/2025_01_22_16_03 |
ttl |
ON |
| 2025_01_23 |
13_20 |
RNAi_ttl_2025/control/ON/2025_01_23_13_20 |
control |
ON |
DS Probe experiments - v2
Probed the direction selectivity of some little studied cell types using the nested_rf_protocol instead of the previous DS_probe_protocol.
Fly strains
| SS00395 |
w+(DL);;pJFRC28-10XUAS-IVS-GFP-p10(attP2) |
TmY3 |
cytoplasmic GFP |
| SS02701 |
w+(DL);;pJFRC28-10XUAS-IVS-GFP-p10(attP2) |
TmY18 |
cytoplasmic GFP |
| SS2594 |
w+(DL);;pJFRC28-10XUAS-IVS-GFP-p10(attP2) |
TmY5a |
cytoplasmic GFP |
| SS03722 |
w+(DL);;pJFRC28-10XUAS-IVS-GFP-p10(attP2) |
Tm5Y |
cytoplasmic GFP |
Experiment log
| 2025_04_08 |
12_51 |
DS_v2/TmY3/2025_04_08_12_51 |
TmY3 |
| 2025_04_11 |
16_20 |
DS_v2/TmY3/2025_04_11_16_20 |
TmY3 |
| 2025_04_14 |
13_57 |
DS_v2/TmY3/2025_04_14_13_57 |
TmY3 |
| 2025_04_15 |
12_31 |
DS_v2/TmY18/2025_04_15_12_31 |
TmY18 |
| 2025_04_16 |
11_29 |
DS_v2/TmY18/2025_04_16_11_29 |
TmY18 |
| 2025_04_18 |
16_26 |
DS_v2/TmY18/2025_04_18_16_26 |
TmY18 |
| 2025_04_22 |
11_58 |
DS_v2/TmY5a/2025_04_22_11_58 |
TmY5a |
| 2025_05_06 |
12_19 |
DS_v2/TmY5a/2025_05_06_12_19 |
TmY5a |
| 2025_05_06 |
15_24 |
DS_v2/TmY5a/2025_05_06_15_24 |
TmY5a |
| 2025_05_08 |
12_19 |
DS_v2/Tm5Y/2025_05_08_12_19 |
Tm5Y |
| 2025_05_09 |
14_56 |
DS_v2/Tm5Y/2025_05_09_14_56 |
Tm5Y |
| 2025_05_09 |
15_46 |
DS_v2/Tm5Y/2025_05_09_15_46 |
Tm5Y |
T4T5 MARCM experiments - Summer2025
These were the first experiments where MARCM flies were used. The MARCM method was used as an alternative to RNAi to manipulate protein expression in T4/T5 cells. The MARCM method is a sparse labelling approach, so not all of the T4/T5 cells will contain the effector with the manipulation and GFP labelling, but the cells that do express GFP and the manipulation can be targeted for recording.
Mid-way through the experiments Jin Yong and Ed realised that the eyes of the flies were lighter than they would have expected, so they switched to using a w+ background flies to improve eye pigmentation and hopefully avoid any potential issues from the reduced eye pigmentation in the earlier w- flies.
The protocol was updated from that used for the RNAi experiments so that the background value was reduced to (4/15) from (6/15) to improve the contrast of the stimuli against the background.
Fly strains
| FRT40A |
hsFlp, 10XUAS-mCD8-GFP; tub-GAL80, FRT40A/CyO; 42F06-GAL4 |
T4/T5 |
MARCM, membrane bound GFP |
| tutl[SK], FRT 40A |
hsFlp, 10XUAS-mCD8-GFP; tub-GAL80, FRT40A/CyO; 42F06-GAL4 |
T4/T5 |
MARCM, membrane bound GFP |
| w+; FRT40A |
hsFlp, 10XUAS-mCD8-GFP; tub-GAL80, FRT40A/CyO; 42F06-GAL4 |
T4/T5 |
MARCM, membrane bound GFP |
| w+; tutl[SK], FRT 40A |
hsFlp, 10XUAS-mCD8-GFP; tub-GAL80, FRT40A/CyO; 42F06-GAL4 |
T4/T5 |
MARCM, membrane bound GFP |
Experiment log
| 2025_07_12 |
17_31 |
T4T5_Summer2025/2025_07_12_17_31 |
0 |
| 2025_07_14 |
16_18 |
T4T5_Summer2025/2025_07_14_16_18 |
0 |
| 2025_07_17 |
14_38 |
T4T5_Summer2025/2025_07_17_14_38 |
0 |
| 2025_07_17 |
15_05 |
T4T5_Summer2025/2025_07_17_15_05 |
0 |
| 2025_07_22 |
16_38 |
T4T5_Summer2025/2025_07_22_16_38 |
0 |
| 2025_07_22 |
18_15 |
T4T5_Summer2025/2025_07_22_18_15 |
0 |
| 2025_07_23 |
13_34 |
T4T5_Summer2025/2025_07_23_13_34 |
0 |
| 2025_07_25 |
16_34 |
T4T5_Summer2025/2025_07_25_16_34 |
0 |
| 2025_07_29 |
14_11 |
T4T5_Summer2025/2025_07_29_14_11 |
0 |
| 2025_08_26 |
14_39 |
T4T5_Summer2025/2025_08_26_14_39 |
0 |
| 2025_08_26 |
16_31 |
T4T5_Summer2025/2025_08_26_16_31 |
0 |
| 2025_08_27 |
12_32 |
T4T5_Summer2025/2025_08_27_12_32 |
0 |
| 2025_08_27 |
16_02 |
T4T5_Summer2025/2025_08_27_16_02 |
0 |
| 2025_08_28 |
14_46 |
T4T5_Summer2025/2025_08_28_14_46 |
0 |
| 2025_09_03 |
11_18 |
T4T5_Summer2025/2025_09_03_11_18 |
0 |
| 2025_09_04 |
13_37 |
T4T5_Summer2025/2025_09_04_13_37 |
0 |
| 2025_09_05 |
15_51 |
T4T5_Summer2025/2025_09_05_15_51 |
0 |
| 2025_09_08 |
15_45 |
T4T5_Summer2025/2025_09_08_15_45 |
0 |
| 2025_09_09 |
12_16 |
T4T5_Summer2025/2025_09_09_12_16 |
0 |
| 2025_09_10 |
14_15 |
T4T5_Summer2025/2025_09_10_14_15 |
1 |
| 2025_09_10 |
16_11 |
T4T5_Summer2025/2025_09_10_16_11 |
1 |
| 2025_09_11 |
11_08 |
T4T5_Summer2025/2025_09_11_11_08 |
1 |
| 2025_09_11 |
12_49 |
T4T5_Summer2025/2025_09_11_12_49 |
1 |
| 2025_09_12 |
12_23 |
T4T5_Summer2025/2025_09_12_12_23 |
1 |
| 2025_09_12 |
12_40 |
T4T5_Summer2025/2025_09_12_12_40 |
1 |
T4T5 MARCM experiments - Autumn2025
Continuation of the MARCM experiments started in the summer. The protocol was adjusted to include bar flashes and an additional speed for the bar sweeps.
Fly strains
| w+; FRT40A |
hsFlp, 10XUAS-mCD8-GFP; tub-GAL80, FRT40A/CyO; 42F06-GAL4 |
T4/T5 |
MARCM, membrane bound GFP |
| w+; tutl[SK], FRT 40A |
hsFlp, 10XUAS-mCD8-GFP; tub-GAL80, FRT40A/CyO; 42F06-GAL4 |
T4/T5 |
MARCM, membrane bound GFP |
Experiment log
| 2025_10_20 |
11_53 |
T4T5_autumn2025/2025_10_20_11_53 |
1 |
| 2025_10_20 |
12_18 |
T4T5_autumn2025/2025_10_20_12_18 |
1 |
| 2025_10_22 |
13_10 |
T4T5_autumn2025/2025_10_22_13_10 |
1 |
| 2025_10_22 |
13_51 |
T4T5_autumn2025/2025_10_22_13_51 |
1 |
| 2025_10_23 |
10_31 |
T4T5_autumn2025/2025_10_23_10_31 |
1 |
| 2025_10_23 |
13_53 |
T4T5_autumn2025/2025_10_23_13_53 |
1 |
| 2025_10_23 |
15_03 |
T4T5_autumn2025/2025_10_23_15_03 |
1 |
| 2025_10_24 |
12_49 |
T4T5_autumn2025/2025_10_24_12_49 |
1 |
| 2025_10_24 |
17_50 |
T4T5_autumn2025/2025_10_24_17_50 |
1 |
| 2025_10_28 |
13_54 |
T4T5_autumn2025/2025_10_28_13_54 |
1 |
| 2025_10_28 |
17_37 |
T4T5_autumn2025/2025_10_28_17_37 |
1 |
| 2025_10_29 |
09_21 |
T4T5_autumn2025/2025_10_29_09_21 |
1 |
| 2025_10_29 |
11_06 |
T4T5_autumn2025/2025_10_29_11_06 |
1 |
| 2025_10_30 |
10_47 |
T4T5_autumn2025/2025_10_30_10_47 |
1 |
| 2025_10_31 |
10_16 |
T4T5_autumn2025/2025_10_31_10_16 |
1 |
| 2025_11_03 |
11_54 |
T4T5_autumn2025/2025_11_03_11_54 |
1 |
| 2025_11_03 |
15_50 |
T4T5_autumn2025/2025_11_03_15_50 |
1 |
| 2025_11_04 |
10_01 |
T4T5_autumn2025/2025_11_04_10_01 |
1 |
| 2025_11_04 |
11_56 |
T4T5_autumn2025/2025_11_04_11_56 |
1 |
| 2025_11_05 |
10_10 |
T4T5_autumn2025/2025_11_05_10_10 |
1 |
| 2025_11_05 |
15_54 |
T4T5_autumn2025/2025_11_05_15_54 |
1 |
| 2025_11_05 |
18_33 |
T4T5_autumn2025/2025_11_05_18_33 |
1 |
| 2025_11_10 |
10_17 |
T4T5_autumn2025/2025_11_10_10_17 |
1 |
| 2025_11_10 |
11_43 |
T4T5_autumn2025/2025_11_10_11_43 |
1 |
| 2025_11_11 |
12_04 |
T4T5_autumn2025/2025_11_11_12_04 |
1 |
| 2025_11_13 |
11_03 |
T4T5_autumn2025/2025_11_13_11_03 |
1 |
| 2025_11_13 |
12_21 |
T4T5_autumn2025/2025_11_13_12_21 |
1 |
| 2025_11_17 |
09_44 |
T4T5_autumn2025/2025_11_17_09_44 |
1 |
CGP54626
Selective GABA-B receptor antagonist CGP54626 was applied to T4 and T5 cells to investigate the role of GABA-B receptor mediated inhibition on their receptive field properties.
The protocol was adjusted again - the square flash stimuli were removed from the second protocol and 2 faster speeds were added to the moving bar sweeps.
Fly strains
| SS02344 |
w+(DL);;pJFRC28-10XUAS-IVS-GFP-p10(attP2) |
T4 |
cytoplasmic GFP |
| SS25175 |
w+(DL);;pJFRC28-10XUAS-IVS-GFP-p10(attP2) |
T5 |
cytoplasmic GFP |
Experiment log
| 2025_11_18 |
10_08 |
CGP54626/T4/pre/2025_11_18_10_08 |
T4 |
pre |
| 2025_11_18 |
10_37 |
CGP54626/T4/post/2025_11_18_10_37 |
T4 |
post |
| 2025_11_19 |
10_59 |
CGP54626/T4/pre/2025_11_19_10_59 |
T4 |
pre |
| 2025_11_19 |
11_34 |
CGP54626/T4/post/2025_11_19_11_34 |
T4 |
post |
| 2025_11_20 |
13_16 |
CGP54626/T4/pre/2025_11_20_13_16 |
T4 |
pre |
| 2025_11_20 |
13_48 |
CGP54626/T4/post/2025_11_20_13_48 |
T4 |
post |
| 2025_11_21 |
09_54 |
CGP54626/T4/pre/2025_11_21_09_54 |
T4 |
pre |
| 2025_11_21 |
10_26 |
CGP54626/T4/post/2025_11_21_10_26 |
T4 |
post |
| 2025_11_21 |
14_57 |
CGP54626/T4/pre/2025_11_21_14_57 |
T4 |
pre |
| 2025_11_21 |
15_29 |
CGP54626/T4/post/2025_11_21_15_29 |
T4 |
post |
| 2025_11_24 |
09_30 |
CGP54626/T5/pre/2025_11_24_09_30 |
T5 |
pre |
| 2025_11_24 |
10_02 |
CGP54626/T5/post/2025_11_24_10_02 |
T5 |
post |
| 2025_11_25 |
10_36 |
CGP54626/T5/pre/2025_11_25_10_36 |
T5 |
pre |
| 2025_11_25 |
11_13 |
CGP54626/T5/post/2025_11_25_11_13 |
T5 |
post |
| 2025_11_25 |
15_52 |
CGP54626/T5/pre/2025_11_25_15_52 |
T5 |
pre |
| 2025_11_25 |
16_24 |
CGP54626/T5/post/2025_11_25_16_24 |
T5 |
post |
| 2025_12_02 |
09_27 |
CGP54626/T5/pre/2025_12_02_09_27 |
T5 |
pre |
| 2025_12_02 |
10_06 |
CGP54626/T5/post/2025_12_02_10_06 |
T5 |
post |
| 2025_12_03 |
09_34 |
CGP54626/T5/pre/2025_12_03_09_34 |
T5 |
pre |
| 2025_12_03 |
10_08 |
CGP54626/T5/post/2025_12_03_10_08 |
T5 |
post |
| 2025_12_03 |
13_32 |
CGP54626/T5/pre/2025_12_03_13_32 |
T5 |
pre |
| 2025_12_03 |
14_09 |
CGP54626/T5/post/2025_12_03_14_09 |
T5 |
post |
| 2025_12_04 |
10_22 |
CGP54626/T4/pre/2025_12_04_10_22 |
T4 |
pre |
| 2025_12_04 |
11_00 |
CGP54626/T4/post/2025_12_04_11_00 |
T4 |
post |
Experiment log - duplicates experiments
| 2025_12_01 |
10_29 |
CGP54626/pre/2025_12_01_10_29 |
pre |
| 2025_12_01 |
11_46 |
CGP54626/pre/2025_12_01_11_46 |
pre |
| 2025_12_01 |
15_18 |
CGP54626/pre/2025_12_01_15_18 |
pre |
| 2025_12_01 |
15_43 |
CGP54626/pre/2025_12_01_15_43 |
pre |
| 2025_12_01 |
16_01 |
CGP54626/pre/2025_12_01_16_01 |
pre |
| 2025_12_02 |
08_59 |
CGP54626/pre/2025_12_02_08_59 |
pre |
| 2025_11_19 |
11_17 |
CGP54626/post/2025_11_19_11_17 |
post |
| 2025_11_19 |
11_51 |
CGP54626/post/2025_11_19_11_51 |
post |
| 2025_11_20 |
14_06 |
CGP54626/post/2025_11_20_14_06 |
post |
| 2025_11_21 |
10_44 |
CGP54626/post/2025_11_21_10_44 |
post |
| 2025_11_21 |
15_46 |
CGP54626/post/2025_11_21_15_46 |
post |
| 2025_11_24 |
10_28 |
CGP54626/post/2025_11_24_10_28 |
post |
Octopamine
The same protocol as used for the CGP54626 experiments was used to investigate the effect of octopamine application on T4 and T5 cell receptive field properties.
Fly strains
| SS02344 |
w+(DL);;pJFRC28-10XUAS-IVS-GFP-p10(attP2) |
T4 |
cytoplasmic GFP |
| SS25175 |
w+(DL);;pJFRC28-10XUAS-IVS-GFP-p10(attP2) |
T5 |
cytoplasmic GFP |
Experiment log
| 2025_12_08 |
10_43 |
Octopamine/pre/2025_12_08_10_43 |
T4 |
pre |
| 2025_12_08 |
11_15 |
Octopamine/post/2025_12_08_11_15 |
T4 |
post |
| 2025_12_09 |
13_17 |
Octopamine/pre/2025_12_09_13_17 |
T4 |
pre |
| 2025_12_09 |
13_51 |
Octopamine/post/2025_12_09_13_51 |
T4 |
post |
| 2025_12_09 |
14_26 |
Octopamine/wash/2025_12_09_14_26 |
T4 |
wash |
| 2025_12_12 |
09_23 |
Octopamine/pre/2025_12_12_09_23 |
T4 |
pre |
| 2025_12_12 |
09_58 |
Octopamine/post/2025_12_12_09_58 |
T4 |
post |
| 2025_12_16 |
12_07 |
Octopamine/pre/2025_12_16_12_07 |
T4 |
pre |
| 2025_12_16 |
12_41 |
Octopamine/post/2025_12_16_12_41 |
T4 |
post |
| 2025_12_17 |
09_18 |
Octopamine/pre/2025_12_17_09_18 |
T4 |
pre |
| 2025_12_17 |
09_57 |
Octopamine/post/2025_12_17_09_57 |
T4 |
post |
| 2025_12_19 |
10_15 |
Octopamine/pre/2025_12_19_10_15 |
T5 |
pre |
| 2025_12_19 |
10_55 |
Octopamine/post/2025_12_19_10_55 |
T5 |
post |
| 2025_12_19 |
11_12 |
Octopamine/post/2025_12_19_11_12 |
T5 |
post |
| 2025_12_22 |
09_33 |
Octopamine/pre/2025_12_22_09_33 |
T5 |
pre |
| 2025_12_22 |
10_08 |
Octopamine/post/2025_12_22_10_08 |
T5 |
post |
Tests with the SS0324 T4T5 line
These experiments were done to test whether the mixed T4/T5 split GAL4 line cells responded differently to the single T4 or T5 split GAL4 lines used previously since some differences in the control / baseline responses of T4/T5 cells were observed when running the CGP54626 experiments.
Fly strains
| SS00324 |
w+(DL);;pJFRC28-10XUAS-IVS-GFP-p10(attP2) |
T4/T5 |
cytoplasmic GFP |
Experiment log
| 2025_12_17 |
14_03 |
SS0324_T4T5/OFF/2025_12_17_14_03 |
OFF |
| 2025_12_17 |
15_01 |
SS0324_T4T5/OFF/2025_12_17_15_01 |
OFF |